The Champagne Artifact in Negative Staining TEM

Stacking in lipid vesicle-tubulin mixtures is an artifact of negative staining

Multilamellar stacking seen in negatively stained lipid vesicle-tubulin mixtures has been attributed to lipid-protein interactions (Caron, J. M., and R. D. Berlin, 1979, J. Cell Biol. 81:665-671). We show that this stacking is produced by the phosphotungstic acid used for staining, independent of the presence of tubulin in the sample. The morphology of negatively stained single bilayer vesicles...

Bipolar staining of gram-negative bacillus in cerebrospinal fluid.

A 36-year-old woman was diagnosed with a sellar-suprasellar craniopharyngioma and underwent transsphenoidal resection of the tumor. A lumbar drain was placed to minimize intracranial pressure and was removed on postoperative day 6. On postoperative day 9, the patient began to leak cerebrospinal fluid (CSF) from her right nostril. A second lumbar drain was placed. One day later, the patient deve...

Convective Mass Transfer in a Champagne Glass

Flow Visualization in engraved champagne tasting glasses

Glass shape and especially his open aperture is suspected to play an important role as concerns the kinetics of CO2 and flavor release during champagne tasting. In recent years, much interest has been devoted to depict each and every parameter involved in the release of gaseous CO2 from glasses poured with champagne. One cannot understand the bubbling and aromatic exhalation events in champagne...

Positive staining with Congo red in tissues with heat artifact due to cautery.

Congo red staining is rarely the source of false positives for amyloid. On the other hand, the heat artifact due to cautery in surgical specimens adopts some hyaline features, which can mimic amyloid deposits many times. Therefore, we decided to investigate the behavior of these artifacted areas with some of the ancillary techniques that are commonly used when diagnosing amyloidosis: Congo red ...